FAQ: Can Endoproteinase LysC be used in acidic buffer conditions?

Yes,  Endoproteinase LysC can be used in acidic buffer conditions. Robust cleavage yielding the products has been observed in the following buffers:

  • 20 mM sodium acetate pH 4.5
  • 20 mM sodium acetate pH 5.5
  • 20 mM sodium acetate pH 6.0
  • 20 mM sodium acetate pH 6.5

Digests can also be run in Tris buffer between a pH range of 7.0 - 9.1.