HomeFAQsWhich strain of Competent E. coli should I use for general cloning?
FAQ: Which strain of Competent E. coli should I use for general cloning?
NEB 5-alpha Competent E. coli (NEB #C2987) are a high efficiency derivative of DH5α™, the industry standard cloning strain. NEB Turbo Competent E. coli (NEB #C2984) and NEB 5-alpha F´Iq Competent e. coli (NEB #C2992) allow potentially toxic genes to be cloned due to tight control of expression by lac Iq and are suitable for blue/white screening. NEB Turbo Competent E. coli (NEB #C2984) bring unmatchable speed to your transformations with visible colonies after just 6.5 hours and plasmid preparation capability after 4 hours. NEB 10-beta Competent e. coli (NEB #C3019) are a derivative of DH10B™ and can be used for transforming large plasmids and BACs. Dam-/dcm- Competent E. coli (NEB #C2925) can be used for methylation-free plasmid growth. NEB Stable Competent E. coli (NEB #C3040) are suitable for cloning and propagation of DNA with repetitive elements as the frequency of recombination is much lower.
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