FAQ: What other materials do I need in addition to the NEBNext Ultra II First Strand Synthesis Module for RNA-seq experiments?

If you are starting from total RNA, we recommend either the NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490) or the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (#E6310) for use prior to first strand synthesis.

For strand-specific RNA-seq workflows, use the following kits:
NEBNext Ultra II Directional RNA Second Strand Synthesis Module (#E7550)
NEBNext Ultra II End Repair/dA-Tailing Module (#E7546)
NEBNext Ultra II Ligation Module (#E7595)
NEBNext Ultra II Q5 Master Mix (#M0544)

For non-directional RNA-seq workflows, use the following kits:
NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (#E6111)
NEBNext Ultra II End Repair/dA-Tailing Module (#E7546)
NEBNext Ultra II Ligation Module (#E7595)
NEBNext Ultra II Q5 Master Mix (#M0544)

For adaptors and primers, NEBNext Oligos (#E7335, #E7550, #E7710, #E7730, #E7600, or #E6609) are compatible, as are adaptors and primers from other sources.