HomeFAQsCan I use other competent E. coli strains than NEB 10-beta? Can I use subcloning efficiency cells?
FAQ: Can I use other competent E. coli strains than NEB 10-beta? Can I use subcloning efficiency cells?
Yes, other cell strains can be used, but large assemblies will require strains
known to maintain large plasmid stability. In our experience NEB 10-beta
competent E. coli (High Efficiency; NEB #C3019) is an excellent choice for
Golden Gate Assembly. For smaller assemblies other strains such as NEB
5-alpha Competent E. coli (High Efficiency; NEB #C2987), NEB Stable Competent
E. coli (High Efficiency; NEB #C3040), NEB Turbo Competent E. coli (High
Efficiency, NEB #C2984) or NEB T7 Express Competent E. coli (High Efficiency;
NEB #C2566) can also be used. Subcloning efficiency cells will result in lower
transformation levels and should not be used for multi-component assemblies.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.