FAQ: Can I use other competent E. coli strains than NEB 10-beta? Can I use subcloning efficiency cells?

Yes, other cell strains can be used, but large assemblies will require strains known to maintain large plasmid stability. In our experience NEB 10-beta competent E. coli (High Efficiency; NEB #C3019) is an excellent choice for Golden Gate Assembly. For smaller assemblies other strains such as NEB 5-alpha Competent E. coli (High Efficiency; NEB #C2987), NEB Stable Competent E. coli (High Efficiency; NEB #C3040), NEB Turbo Competent E. coli (High Efficiency, NEB #C2984) or NEB T7 Express Competent E. coli (High Efficiency; NEB #C2566) can also be used. Subcloning efficiency cells will result in lower transformation levels and should not be used for multi-component assemblies.