- Incorrect ratio of dCas9 to guide RNA, and target site-For complete binding we recommend a 10:10:1 or higher molar ratio (SNAP dCas9 NLS : guide RNA : target site).
- Suboptimal guide RNA sequence-Verify the sequence and design of the guide RNA.
- Poor quality guide RNA-Verify the integrity of the guide RNA by gel electrophoresis.
- Buffer incompatibility-Please use the 10X NEBuffer 3.1 included with the enzyme.
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
