HomeFAQsWhat polymerases can be used to amplify the fragments that will be phosphorylated and ligated by the KLD mix?
FAQ: What polymerases can be used to amplify the fragments that will be phosphorylated and ligated by the KLD mix?
The KLD mix is to be used with fragments that have been amplified with Q5 High Fidelity polymerase or Phusion High Fidelity polymerase. This product is not suitable for amplicons with 3´ overhangs (ex: Taq amplicons). We do not recommend the use of this product on DNA fragments that have been amplified by other polymerases.
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