HomeFAQsWhat temperature should I use for cDNA synthesis with Luna® RT-qPCR kits?
FAQ: What temperature should I use for cDNA synthesis with Luna® RT-qPCR kits?
The thermostable Luna WarmStart® Reverse Transcriptase supports efficient cDNA synthesis at elevated temperatures compared to MMLV. In general, cDNA synthesis can be accomplished with a 10 minute incubation at 55°C. For difficult templates, the cDNA synthesis temperature can be increased to 60°C.