HomeFAQsHow much primer and probe should I use with the Luna® Universal Probe RT-qPCR Kit?
FAQ: How much primer and probe should I use with the Luna® Universal Probe RT-qPCR Kit?
For most targets, a final concentration of 400 nM for each primer and 200 nM for the probe will provide optimum performance. If needed, the final primer concentration can be optimized between 100–900 nM and the final probe concentration can be optimized between 100-500 nM.
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