HomeFAQsCan I set up my Luna® qPCR at room temperature?
FAQ: Can I set up my Luna® qPCR at room temperature?
Yes. Luna qPCR can be set up at room temperature, but for best results, reactions should be kept on ice prior to thermocycling. The DNA polymerase present in the qPCR Master Mix, NEB’s Hot Start Taq DNA Polymerase (NEB #M0495), contains a unique modified aptamer (SOMAmer®) for temperature-dependent activity control, and is inhibited below 45°C. This reversible inhibition prevents undesired nonspecific activity until the reaction is heated above the release temperature during the initial denaturation step of PCR cycling.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.