FAQ: What components are contained in the WarmStart® Colorimetric LAMP 2X Master Mix and what else do I need to perform a LAMP reaction?

The WarmStart Colorimetric 2X Master Mix contains a low-Tris reaction buffer with all necessary cofactors at optimized 2X concentrations for LAMP, Bst 2.0 WarmStart DNA Polymerase, WarmStart RTx and Phenol Red for pH detection of LAMP. To perform a LAMP or RT-LAMP reaction, the user adds primers, target DNA or RNA and adjusts total reaction volume to a final concentration of 1X. Detailed instructions can be found in the Mix Manual.