FAQ: How do I design LAMP Primers?

LAMP primers can be challenging to design manually, and software programs are strongly recommended for both ease of design and likelihood of reaction success. We recommend using the NEB LAMP Primer Design Tool. As performance and levels of non-template amplification can vary even with in silico design, we recommend evaluating 2–4 complete sets of LAMP primers for optimal sensitivity and specificity before choosing a final set.  For an overview of how LAMP primers are designed and utilized, watch this LAMP explainer video.
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    Loop Mediated Isothermal Amplification (LAMP) Tutorial

    Did you know that this isothermal amplification method can be performed in as little as 5-10 minutes with limited resources? Get a quick overview on how LAMP works in this animation.