FAQ: What is the difference between PNGase F and PNGase A?

PNGase F and PNGase A both cleave between the innermost GlcNAc and asparagine residues of N-linked glycans on both glycoproteins and glycopeptides. PNGase F can cleave almost all N-linked glycans from high mannose, hybrid, and complex oligosaccharides. However, PNGase F cannot cleave N-glycans with core α1-3 fucosylation.  In contrast, PNGase A cleaves N-linked glycans from high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without α(1,3)-linked core fucose residues. 

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PNGase A