FAQ: How long should I incubate the Colorimetric LAMP reaction before checking the results?

Our recommended incubation time for Colorimetric LAMP is 30 min. With this time, most positive LAMP reactions will have completed with no amplification in non-template controls. If desired, the time can be shortened to 15–20 minutes with good assays or high copy targets, or lengthened to 60 minutes with very low input, impure samples, and slower assays. If optimizing reaction time, progress can be simply checked by looking at the reaction colors and returning vessels back to incubation if incomplete.