FAQ: What types of input samples/materials are compatible with the LAMP/RT-LAMP mixes?

LAMP is typically a robust and inhibitor tolerant technique and the WarmStart® Multi-Purpose LAMP/RT-LAMP Master Mix is capable of direct amplification using a variety of sample types. For highest specificity and sensitivity, we recommend purified nucleic acid as input. Most routine methods of template purification are sufficient, such as the Monarch® Nucleic Acid Extraction Kits. We suggest using 2 µl of extracted nucleic acid for a 25 µl LAMP reaction. Larger or smaller volumes of samples may be tolerated. Samples in transport media (VTM or UTM) should be kept to less than 2 µl (8% v/v).