FAQ: Which alkaline phosphatase, Quick CIP, from the Quick Dephosphorylation Kit, rSAP, CIP or Antarctic Phosphatase works best?

Quick CIP (NEB #M0508) and rSAP (NEB #M0371) are superior choices because they combine the advantages of both CIP (NEB #M0290) and Antarctic Phosphatase (NEB #M0289). The Quick Dephosphorylation Kit additionally offers a quick protocol. Quick CIP, rSAP and CIP have high specific activities, however, CIP cannot be completely heat-inactivated. Quick CIP and rSAP are preferred over Antarctic Phosphatase because they do not require added zinc or any other co-factors in the reaction buffer. As a result, Quick CIP and rSAP can be added directly to restriction enzyme digests. Additionally, after heat-inactivation of these enzymes, it is not necessary to purify vector DNA prior to the ligation reaction.