Home FAQs Is it necessary to add a “G” into the oligo following the T7 promoter sequence?

FAQ: Is it necessary to add a “G” into the oligo following the T7 promoter sequence?

At least one G, directly downstream of the “TATA” of the T7 promoter sequence is required for efficient transcription.  This G will be the +1 of the transcript.  If the 20 nucleotide target-specific sequence selected has a G at the 5’ end of the 20 nucleotides then it is not necessary to add an additional G as the G within the target-specific sequence will serve as the +1.