FAQ: How long can I store the master mix after adding the primers?

Once Quant Kit Primers are added to the master mix, the mix and primers solution is stable for 6 weeks at 4°C for convenience. If you anticipate longer times between runs, store at –20°C for up to 7 months. The mix and primers will be stable for 30 freeze/thaw cycles. With ROX added, master mix is stable at –20°C for up to 7 months and over 30 freeze/thaw cycles.