HomeFAQsCan I see adaptor dimers as a measure of library quality?
FAQ: Can I see adaptor dimers as a measure of library quality?
Yes. While a melt curve is not required for the kit, and omitting it will reduce run times, data from the melt analysis can be used for library quality information. When adaptor dimers are present at >5% of the library sample, a single, sharp melt peak can be observed at 81.5 °C. In all other cases, a broad or multi-peaked melt curve will be produced, indicative of the range and diversity of the library being amplified.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.