FAQ: How much should I dilute my library to run in the Quant Kit?

We recommend an initial 1:1,000 dilution of the library using the provided Library Dilution Buffer. For most library preps this will result in >10 pM library, so we recommend two more 1:10 serial dilutions resulting in final 1:10,000 and 1:100,000 dilutions. For new protocols and libraries we recommend running both of these dilutions in the Quant Kit. With more established workflows or to maximize the number of libraries quantitated, it is often possible to use only the 1:100,000 dilution for quantitation. If custom dilutions are desired, please ensure that final diluted libraries fall within the range of the standards, 10 pM – 10 fM. If you need dilution buffer in addition to what is provided in the kit, it can be purchased separately.