FAQ: Can I run the NEBNext Library Quant Kit on my qPCR machine?

The Quant Kit is compatible with all qPCR instruments tested thus far, using either standard or fast temperature ramping protocols. For machines that use a reference dye for normalization, please consult the kit manual or the FAQ on ROX for appropriate ROX selection. The Quant Kit has been run successfully on the following qPCR machines:


Manufacturer Instrument
Thermo-Fisher®  StepOne
StepOnePlus
Prism® 7000
Prism® 7900HT
7300, 7500, 7500 Fast
QuantStudio 6
QuantStudio 7
QuantStudio 12K Flex
ViiA 7
Bio-Rad iQ® 5
CFXConnect
CFX96
CFX384
Roche LightCycler® 96
LightCycler® 480
LightCycler® 2.0
Agilent Mx3000p
Mx4500P
Aria Mx
Qiagen  Rotorgene® Q
Illumina Eco

Please contact us at [email protected] if you would like to discuss performance on any qPCR instrument not listed above.