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HomeFAQsCan I analyze protein glycosylation with only benchtop instrumentation and reagents?
FAQ: Can I analyze protein glycosylation with only benchtop instrumentation and reagents?
Since loss of sugars alters the molecular weight of a protein, the simpler method to determine whether a protein is glycosylated is to follow its migration by SDS-PAGE, before and after deglycosylation (with one or more glycosidases of different specificities).
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