FAQ: What is the largest single fragment that has been assembled with NEBuilder HiFi DNA Assembly Master Mix?

NEBuilder HiFi DNA Assembly Master Mix has been used to clone a 12 kb DNA fragment into a 7.4 kb plasmid in E. coli, totaling up to 19 kb in length. For assembled products greater than 15 kb, NEB recommends NEB 10-beta Competent E. coli (High Efficiency, NEB #C3019) which are included in the NEBuilder HiFi DNA Assembly Bundle for Large Fragments (NEB #E2623) (include link). Alternatively, you can use NEB 10-beta Electrocompetent E. coli (NEB #C3020).