FAQ: What are the advantages of this method compared to traditional cloning methods?

NEBuilder HiFi DNA assembly allows insertion of one or more DNA fragments into virtually any position of the linearized vector and does not rely on the presence of restriction sites within a particular sequence. Therefore, the user has complete control over what is assembled and insertion of unwanted additional sequence, often used to facilitate the manipulation of multiple DNA sequences, can be avoided. Furthermore, the NEBuilder HiFi DNA assembly method is fast, relative to standard restriction enzyme-based cloning. Lastly, a greater number of DNA fragments can be joined in a single reaction with greater efficiency than conventional methods.