FAQ: Is this method applicable to the assembly of repetitive sequences?

Yes. However, one must ensure that each DNA fragment includes a unique overlap so that the sequences may anneal and are properly arranged. The repetitive sequence can also be internalized in the first stage of a two-stage assembly strategy. If having repetitive sequences at the ends of each fragment is unavoidable, the correct DNA assembly may be produced, albeit at lower efficiency than other, unintended assemblies.
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    NEBuilder Assembly Tool 2.0 What’s New?

    NEBuilder Assembly Tool can be used to design primers for your NEBuilder HiFi or Gibson Assembly reactions based on entered fragment sequences and the polymerase being used for amplification. This video will highlight some useful updates and the main differences between the two versions.