FAQ: How do I use WarmStart® RTx in RT-LAMP?

Prepare a 25 µL mix containing:

2.5 µL Isothermal Amplification Buffer (1X)
6 mM MgSO4 (8 mM total)
1.4 mM dNTP
1.6 µM FIP/BIP, 0.2 µM F3/B3, 0.4 µM LoopF/B
8 U Bst 2.0 WarmStart or Bst 2.0
0.5 µL WarmStart® RTx

Mix, place directly at 65°C