FAQ: How do I use WarmStart® RTx for cDNA synthesis?

Prepare a 20 µL mix containing:

2 µL Isothermal Amplification Buffer (1X)
0.5 mM dNTP
6 µM Random Primer Mix
20 U Murine RNase Inhibitor
0.25 µL WarmStart® RTx

Mix, incubate 5 min at 25°C for annealing and 10 min at 55°C for synthesis. Inactivate RTx with 10 min at 80°C.