Home FAQs Can I use β-N-Acetylglucosaminidase S in a double digest with other exoglycosidases and/or endoglycosidases?

FAQ: Can I use β-N-Acetylglucosaminidase S in a double digest with other exoglycosidases and/or endoglycosidases?

Yes. We recommend double digesting β-N-Acetylglucosaminidase S with other exoglycosidases using 1X GlycoBuffer 1 (50mM NaAcetate, pH 5.5, 5mM CaCl2). We recommend using 1X G7 Reaction Buffer (50 mM sodium phosphate, pH 7.5) for digestion of β-N-Acetylglucosaminidase S with PNGase F and/or O-Glycosidase.