HomeFAQsWill the 5'→3' flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
FAQ: Will the 5'→3' flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
No. The exonuclease will only degrade double- stranded DNA that it encounters while extending a DNA fragment. It will degrade a secondary primer or probe if bound to the same strand (e.g. a mutagenesis primer).