HomeFAQsWhen should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
FAQ: When should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
It is an excellent choice for routine primer extensions
throughout a wide range of template sources. When cost per reaction and yield
are priorities, Hot Start Taq DNA Polymerase is the industry standard.
It is limited by its relatively low thermostability (half-life of 45 minutes at
94°C) and lack of a proofreading exonuclease domain. These characteristics make
it a poor choice for work on long (>5 kb) extensions, on templates with a
high degree of secondary structure, or in applications that strive to minimize
error incorporation. OneTaq® Hot Start DNA Polymerase (NEB #M0481) is recommended for difficult amplicons.
Q5® Hot Start High-Fidelity DNA Polymerase (NEB
#M0493) is recommended for applications requiring high fidelity. LongAmp®
Hot Start Taq DNA Polymerase (NEB
#M0534) is recommended for amplification of long products.