FAQ: What is the difference between Endo H and Endo Hf?

Endo H and Endo Hf are the same enzyme, but Endo Hf is the fusion protein of Endo H and MBP. The clone was engineered with the MBP attached to help in the purification of the enzyme. The fusion protein has identical activity to the non-fusion clone. There is no difference in activity between Endo H and Endo Hf on a glycoprotein.