FAQ: What kind of RNA can be methylated using the mRNA Cap 2'-O-Methyltransferase?

mRNA Cap 2'-O-Methyltransferase requires capped RNA as substrate. Capped RNA can be prepared via in-vitro transcription (T7 High Yield RNA Synthesis Kit, NEB# E2040) using cap analog (ARCA 3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog, NEB# S1411) or by enzymatic capping using the Vaccinia capping enzyme (NEB# M2080).