FAQ: How should I set up a PCR using OneTaq® Hot Start DNA Polymerase?

The general guidelines for a 50 µl reaction are:
1.25 units OneTaq Hot Start DNA Polymerase
200 μM each dNTP
0.2 μM each primer
1-1000 pg plasmid or 1-1000 ng genomic template
1 X Standard Buffer (GC Reaction Buffer can be used for GC-rich amplicons)
Denature at 94°C (no separate activation step is required for the aptamer-based Hot Start enzyme)
Extend 1 minute/kb at 68°C
Reactions can be assembled at room temperature.