HomeFAQsCan DNA Polymerase I (E. coli) be used in other NEBuffers, including CutSmart?
FAQ: Can DNA Polymerase I (E. coli) be used in other NEBuffers, including CutSmart?
DNA Polymerase I (E. coli) is active in NEBuffers 1.1, 2.1, 3.1 and Cutsmart, as well as NEBuffers 1-4, however, optimal activity is observed in NEBuffer 2. All NEBuffers must be supplemented with dNTPs. To see its % functional activity in Cutsmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.