FAQ: Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?

Yes, NEBNext Multiplex Oligos (Adaptors and Primers) for Illumina are compatible with NEBNext kits for DNA, RNA, and ChIP-seq library preparation. Refer to the NEBNext Multiplex Oligos Selection Chart (www.neb.com/oligos)  for the full range of options available. Note: Multiplexing (indexing) oligos are not included in most NEBNext library prep kits, and must be ordered separately.

Adaptors and primers are included in the NEBNext Enzymatic Methyl-seq Kit and the NEBNext Small RNA Library Prep Kits, as they are optimized for specific input types and sample amounts.