HomeFAQsWhat competent cell E. coli strains are suitable for propagating SNAP-tag plasmids?
FAQ: What competent cell E. coli strains are suitable for propagating SNAP-tag plasmids?
NEB 10-beta E. coli (High Efficiency) (NEB #C3019) is recommended for propagating or subcloning. However, when using a ClaI site, dam-/dcm- Competent E. coli (NEB #C2925) is recommended. ClaI is methylation sensitive.