HomeFAQsCan SNAP-tag fusions be purified and refolded from inclusion bodies?
FAQ: Can SNAP-tag fusions be purified and refolded from inclusion bodies?
A SNAP-tag fusion protein has been successfully solubilized from inclusion bodies in bacteria and refolded to yield SNAP-tag capable of reacting with its substrate. (Krayl et al., Anal. Biochemistry, 2006.)