Home FAQs When doing bisulfite treatments of templates, the subsequent PCR reactions can pose a problem as the DNA seems to be very labile after the treatment. Can the PreCR Repair Mix improve these PCR results?

FAQ: When doing bisulfite treatments of templates, the subsequent PCR reactions can pose a problem as the DNA seems to be very labile after the treatment. Can the PreCR Repair Mix improve these PCR results?

Our current mix has UDG in it and won't repair bisulfite-treated DNA templates. Leaving UDG out is a good idea, but for bisulfite sequencing, the DNA has to go through single-strand DNA step, and our current PreCR Repair Mix does not repair ssDNA sufficiently.