Home FAQs I've blunted my sonicated gDNA for 15 minutes instead of the recommended 30 minute incubation time, will the reaction still work?

FAQ: I've blunted my sonicated gDNA for 15 minutes instead of the recommended 30 minute incubation time, will the reaction still work?

A slightly lower transformation efficiency is seen with shorter incubations but the reaction will still work. In general, 1.5 X fewer transformants are seen when PCR product or sheared/nebulized DNA is incubated for 15 minutes instead of 30 minutes. If a 15 minute incubation time is desired, increase blunting mix to 2 µl in the reaction.