FAQ: Can I save the unused portion of the CLuc assay solution?

Ideally the CLuc assay solution is prepared immediately before performing the assays. However, the unused portion of the assay solution can be stored at -20°C for up to 2 days. It must be completely thawed to room temperature (protected from light) and mixed well (do not vortex) before use. Using the previously frozen assay solution will result in approximately a 20% decrease in CLuc activity. The unused portion of the CLuc assay solution should be discarded after one round of freeze-and-thaw.