FAQ: Does BaeI have any special requirements?

BaeI (NEB #R0613) requires S-adenosylmethionine (SAM) for optimal activity (supplied with enzyme as a separate vial).  The enzyme works in a wide range of SAM concentrations so it is not critical to pipette the exact about of SAM into the reaction.  To be more accurate, SAM can be diluted 10x with 1x reaction buffer and then pipetted into the final BaeI reaction.

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BaeI