HomeFAQsWhat buffer should be used with RecAf Protein?
FAQ: What buffer should be used with RecAf Protein?
The supplied RecAf buffer can be used to form stable triple helixes when supplemented with ATP gamma-S. ATP gamma-S is not supplied. Sigma product (A1388) ATP gamma-S can be used. A buffer similar to several published in the scientific literature, 25 mM Tris-acetate (pH 7.8), 4 mM Mg+2 acetate, 0.3 mM ATP gamma-S, 0.4 mM DTT, has been successfully used with the RecAf functional assay.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.