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HomeFAQsWhat should be considered if the methylation using Dnmt1 is not going to completion?
FAQ: What should be considered if the methylation using Dnmt1 is not going to completion?
Dnmt1 loses about 50% activity in 10 months. The SAM is also labile and should be replaced if more than 6 months old. If the stock solution of Dnmt1 is older than 10 months it should be replaced. Tritiated SAM loses about 5% activity per week.