HomeFAQsWill the buffer from the SAM inhibit my reaction?
FAQ: Will the buffer from the SAM inhibit my reaction?
The concentration of SAM is 32 mM. Most often it is used at 80 μM which is a 1:400 dilution. At this level the SAM buffer has little effect on subsequent reactions. SssI Methylase shows full activity at SAM concentrations of 320 μM, a 1:100 dilution.