FAQ: Will Phusion® High-Fidelity DNA Polymerase incorporate dUTPs?

No.Q5U® Hot Start High-Fidelity DNA Polymerase (NEB #M0515) or EpiMark® Hot Start Taq DNA Polymerase (NEB# M0490) would be recommended, as both are able to incorporate dUTP and amplify uracil-containing templates.

Phusion® is a registered trademark of Thermo Fisher Scientific.

EpiMark® is a registered trademark of New England Biolabs, Inc.