FAQ: What is the optimal heat shock time for this strain (NEB #C2987H and NEB #C2987I)?

Heat shock at 42ºC for 30 seconds results in the highest transformation efficiency for NEB 5-alpha competent E.coli (NEB #C2987H and NEB #C2987I). Expect approximately 60% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).