HomeFAQsWhat is the optimal heat shock time for this strain (NEB #C2925H and NEB #C2925I)?
FAQ: What is the optimal heat shock time for this strain (NEB #C2925H and NEB #C2925I)?
Heat shock at 42ºC for 20-30 seconds results in the highest transformation efficiency for dam-/dcm- competent E.coli (NEB #C2925H and NEB #C2925I). The transformation efficiency is not very sensitive to the changing the heat shock time within the range of 20-60 seconds. Expect approximately 20% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).