HomeFAQsIs there a compatible buffer for digesting with Trypsin (P8101S) and Endoproteinase GluC (P8100S) together?
FAQ: Is there a compatible buffer for digesting with Trypsin (P8101S) and Endoproteinase GluC (P8100S) together?
We would suggest a 50:50 mix of each enzyme's buffer. GluC works at maximal activity with a stimulating glutamic acid dipeptide in the reaction. Calcium chloride reduces autolysis of tryspin. Both buffers have a pH 8, Tris-HCI backbone. We have done our GluC/Trypsin digests this way with incubation overnight at 37°C. Neither enzyme is inhibited by the buffer mixing but they will, of course digest each other to some extent.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.