FAQ: Is SacI affected by methylation?

Methylation from common E.coli strains and mammalian sources will not block SacI. AluI methylase (AGmCT), NEB catalog #M0220, can be used to block SacI. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE.

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SacI