HomeFAQsHow long should I incubate cells on ice after DNA has been added (NEB #C2988J)?
FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2988J)?
Incubating DNA with NEB 5-alpha competent cells (Subcloning Efficiency) on ice for 30 minutes is recommended. Expect approximately 30% loss in transformation efficiency when incubating for 10 minutes. (see Figure on the main product page).