Home FAQs Can Klenow Fragment (3'→5' exo-) be used in labeling reactions and partial fill in reactions?

FAQ: Can Klenow Fragment (3'→5' exo-) be used in labeling reactions and partial fill in reactions?

Yes, it is the enzyme of choice for these applications. The lack of 3'→5' exonuclease activity prevents the labeled nucleotides from being removed after they are incorporated by the polymerase.